Codon Usage Frequency Calculator | Analyze DNA Codon Bias

The Codon Usage Frequency Calculator is an essential tool for geneticists, molecular biologists, and bioinformatics professionals. It helps in analyzing the frequency of specific codons within a DNA sequence, which can be crucial for understanding gene expression, protein synthesis, and evolutionary biology.

Codon Usage Frequency Calculator – Analyze and Visualize DNA Codon Bias Instantly

DNA Sequence Input *

Enter a coding DNA sequence (no spaces or numbers). Only A, T, C, G characters are accepted. Sequence length should be a multiple of 3 for accurate codon analysis.

Reading Frame Frame 1 (start at position 1) Frame 2 (start at position 2) Frame 3 (start at position 3)

Choose the reading frame to start codon grouping.

Highlight Specific Codon (optional)

Enter a codon (e.g., ATG) to highlight its frequency in the results.

Reference Organism (optional) None (analyze only your input) Human (Homo sapiens) E. coli (Bacteria) Yeast (S. cerevisiae) Arabidopsis (Plant)

Compare your codon usage to typical frequencies in a reference organism.

Sequence Case Auto-detect Convert to UPPERCASE Convert to lowercase

Choose how to handle sequence letter case.

Example Presets:

Human Hemoglobin Beta (Frame 1, ATG highlight, Human) E. coli lacZ (Frame 1, GAA highlight, E. coli) Yeast ADH1 (Frame 2, TGG highlight, Yeast) Arabidopsis RuBisCO (Frame 1, CCA highlight, Arabidopsis) Random GC-rich (Frame 3, CGG highlight, None)

Calculate Reset

Analyzing sequence…

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